Screening and Identification of an Antagonistic Bacterium Against Tomato Early Blight and Analysis of Its Antimicrobial Peptide Synthesis Genes

YU Hang; WANG Guodong; LI Quansheng

您当前的位置：

首页 >

文章列表页 >

Screening and Identification of an Antagonistic Bacterium Against Tomato Early Blight and Analysis of Its Antimicrobial Peptide Synthesis Genes

更新时间：2026-01-12

- Screening and Identification of an Antagonistic Bacterium Against Tomato Early Blight and Analysis of Its Antimicrobial Peptide Synthesis Genes
- Vol. 40, Issue 1, Pages: 48-57(2025)
- 作者机构：
  
  1. 新疆农垦科学院科研管理处,新疆,石河子,832000
  2. 新疆农垦科学院农田水利与土壤肥料研究所,新疆,石河子,832000
  3. 农业农村部西北绿洲节水农业重点实验室/水肥资源高效利用兵团重点实验室,新疆,石河子,832000
- 作者简介：
- 基金信息：
  
  Science and Technology Program of XPCC (2016AD029);Corps Talent Support Program Youth Project of XPCC (2024)
- DOI：
  CLC： Q933
- Received：23 May 2025，
  
  Published：2025
- 稿件说明：
移动端阅览
于航，王国栋，李全胜．一株番茄早疫病拮抗菌筛选鉴定及其抗菌脂肽合成基因分析[J]．新疆大学学报（自然科学版汉文、维吾尔文），2025，40(1)：48-57．

YU Hang，WANG Guodong，LI Quansheng．Screening and identification of an antagonistic bacterium against tomato early blight and analysis of its antimicrobial peptide synthesis genes[J]．Journal of Xinjiang University(Natural Science Edition in Chinese and Uyghur)，2025，40(1)：48-57．
于航，王国栋，李全胜．一株番茄早疫病拮抗菌筛选鉴定及其抗菌脂肽合成基因分析[J]．新疆大学学报（自然科学版汉文、维吾尔文），2025，40(1)：48-57． DOI：

YU Hang，WANG Guodong，LI Quansheng．Screening and identification of an antagonistic bacterium against tomato early blight and analysis of its antimicrobial peptide synthesis genes[J]．Journal of Xinjiang University(Natural Science Edition in Chinese and Uyghur)，2025，40(1)：48-57． DOI：

摘要

新疆加工番茄产业地位重要，但番茄早疫病频发严重制约其发展．随着绿色健康可持续农业发展的需求，生物防治成为研究热点．采用系列稀释涂布法从新疆长期连作番茄农田的根际土壤中分离细菌，利用平板对峙法筛选番茄早疫病拮抗细菌，经过形态学观察、生理生化实验及16S rDNA基因序列分析鉴定菌株属种分类，采用PCR扩增法检测其抗菌肽合成基因，获得一株对番茄早疫病菌（Alternaria solani）高效拮抗菌株KM432，其抑菌率达61.45%，可在pH 9.0和6%氯化钠条件下生长．菌株KM432含有srfAA、ituC 、bacA、bmyB共4种抗菌肽合成基因．以上结果表明菌株KM432兼具耐盐碱和拮抗病原真菌功能，有在番茄病害绿色防控中应用的潜力．

Abstract

The processing tomato industry in Xinjiang holds significant importance

but the frequent occurrence of early blight severely hampers its development. With the growing demand for green

healthy

and sustainable agriculture

biological control has become a research hotspot. In this study

bacteria are isolated from the rhizosphere soil of tomatoes in long-term continuous cropping fields in Xinjiang using a serial dilution plating method. Antagonistic bacteria against tomato early blight are screened using a dual-culture plate assay. The isolated strain is identified to genus and species level through morphological observation

physiological and biochemical tests

and 16S rDNA gene sequence analysis. Their antimicrobial peptide synthesis genes were detected using PCR amplification. A highly effective antagonistic strain

KM432

against Alternaria solani (the pathogen causing tomato early blight) is obtained

with an inhibition rate of 61.45%. This strain can grow under conditions of pH 9.0 and 6% NaCl. Strain KM432 possesses four antimicrobial peptide synthesis genes: srfAA

ituC

bacA

and bmyB. These results indicate that strain KM432 combines saline-alkali tolerance with pathogenic fungus antagonism

demonstrating potential for application in the green management of tomato diseases.

关键词

Keywords

references

江应红，宋玉兰，赵鑫，等．新疆蔬菜产业发展现状分析及对策建议[J]．中国蔬菜，2024(11)：7-12．

JIANG Y H，SONG Y L，ZHAO X，et al．Current situation of vegetable industry development in Xinjiang and countermeasures[J]．China Vegetables，2024(11)：7-12．(in Chinese)

李荣霞，刘磊，刘伟，等．新疆加工番茄种植现状、问题及建议[J]．中国蔬菜，2022(4)：4-8．

LI R X，LIU L，LIU W，et al．Present situation，problems and suggestions of processing tomato planting in Xinjiang[J]．China Vegetables，2022(4)：4-8．(in Chinese)

郭开发，陈荣毅，赵思峰，等．新疆加工番茄早疫病发生因素的初步分析[J]．中国农学通报，2011，27(25)：142-146．

GUO K F，CHEN R Y，ZHAO S F，et al．The preliminary analysis on occurring factor of processing tomato early blight in Xinjiang[J]．Chinese Agricultural Science Bulletin，2011，27(25)：142-146．(in Chinese)

林青，史应武，王娜，等．加工番茄系统抗性诱导促生菌的筛选鉴定及其促生防病效果[J]．新疆农业科学，2022，59(6)：1466-1474．

LIN Q，SHI Y W，WANG N，et al．Screening and identification of systemic resistant inducing growth-promoting bacteria and their effects on growth and disease prevention of processing tomatoes[J]．Xinjiang Agricultural Sciences，2022，59(6)：1466-1474．(in Chinese)

赵坚，鲍浩，张艳．番茄早疫病可见光图像识别模型研究[J]．江苏农业科学，2024，52(12)：209-217．

ZHAO J，BAO H，ZHANG Y．Study on visible light image recognition model of tomato leaf early blight[J]．Jiangsu Agricultural Sciences，2024，52(12)：209-217．(in Chinese)

王莹莹，纪明山，李宝聚．番茄早疫病病原菌鉴定及综合防治技术[J]．中国蔬菜，2016(1)：85-87．

WANG Y Y，JI M S，LI B J．Identification of pathogen of tomato early blight and its integrated control techniques[J]．China Vegetables，2016(1)：85-87．(in Chinese)

刘升学，李思琪，王晓东，等．番茄早疫病菌dsRNA多样性分析[J]．新疆农业科学，2023，60(12)：3057-3064．

LIU S X，LI S Q，WANG X D，et al．Diversity analysis of dsRNA in Alternaria solani[J]．Xinjiang Agricultural Sciences，2023，60(12)：3057-3064．(in Chinese)

张礼生，陈红印．生物防治作用物研发与应用的进展[J]．中国生物防治学报，2014，30(5)：581-586．

ZHANG L S，CHEN H Y．Advances in research and application of biological control agents in China[J]．Chinese Journal of Biological Control，2014，30(5)：581-586．(in Chinese)

李道明，王瑛，陈超，等．芽孢杆菌几种重要抗菌脂肽研究进展[J]．生物工程学报，2022，38(5)：1768-1783．

LI D M，WANG Y，CHEN C，et al．Advances in several important antimicrobial lipopeptids from Bacillus spp.[J]．Chinese Journal of Biotechnology，2022，38(5)：1768-1783．(in Chinese)

曲晓旭，刘洪霞，高玲，等．芽孢杆菌产抗菌脂肽调控基因快速检测[J]．南京农业大学学报，2016，39(5)：858-864．

QU X X，LIU H X，GAO L，et al．Rapid identification of the encoding genes of antimicrobial lipopeptides producted by Bacillus[J]．Journal of Nanjing Agricultural University，2016，39(5)：858-864．(in Chinese)

叶敏，毛雪，田小卫．黄瓜枯萎病拮抗昆虫共生菌的筛选及抑菌机制初步研究[J]．江苏农业科学，2024，52(19)：143-150．

YE M，MAO X，TIAN X W．Screening of antagonistic insect symbiotic bacteria of cucumber fusarium wilt and preliminary study on its inhibitory mechanism[J]．Jiangsu Agricultural Sciences，2024，52(19)：143-150．(in Chinese)

张丝雨，高文静，张丙翠，等．甲基营养型芽胞杆菌NKG-1对番茄灰霉病的防治效果及全基因组分析[J]．中国生物防治学报，2024，40(3)：679-689．

ZHANG S Y，GAO W J，ZHANG B C，et al．Control effect of Bacillus methylotrophicus NKG-1 on tomato gray and its genome-wide analysis[J]．Chinese Journal of Biological Control，2024，40(3)：679-689．(in Chinese)

王超，郭坚华，席运官，等．拮抗细菌在植物病害生物防治中应用的研究进展[J]．江苏农业科学，2017，45(18)：1-6．

WANG C，GUO J H，XI Y G，et al．Research progress on application of antagonistic bacteria in biological control of plant diseases[J]．Jiangsu Agricultural Sciences，2017，45(18)：1-6．(in Chinese)

刘海洋，王伟，张仁福，等．利用生防菌防治棉花黄萎病效果的制约因素[J]．新疆农业科学，2022，59(1)：155-161．

LIU H Y，WANG W，ZHANG R F，et al．A brief analysis of the factors restricting the effectiveness of controlling cotton Verticillium wilt by using biocontrol bacteria in the field[J]．Xinjiang Agricultural Sciences，2022，59(1)：155-161．(in Chinese)

赵美荣，李永春，张志超．1株拮抗禾谷镰刀菌生防菌株的筛选鉴定[J]．江苏农业科学，2024，52(11)：128-133．

ZHAO M R，LI Y C，ZHANG Z C．Screening and identification of a biocontrol strain against Fusarium graminearum[J]．Jiangsu gricultural Sciences，2024，52(11)：128-133．(in Chinese)

朱旭芬．现代微生物学实验技术[M]．杭州：浙江大学出版社，2011：10-16．

ZHU X F．Modern experimental technique of microbiology[M]．Hangzhou：Zhejiang University Press，2011：10-16．(in Chinese)

BUCHANAN R E，GIBBONS N E（著）．中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组（译）．伯杰细菌鉴定手册（第八版）[M]．北京：科学出版社，1984：729-795．

BUCHANAN R E，GIBBONS N E (Writer)．Translation Team of Bergey’s manual of determinative bacteriology，Institute of Microbiology，Chinese Academy of Sciences (Translator)．Bergey’s manual of determinative bacteriology (8th Edition)[M]．Beijing：China Science Press，1984：729-795．(in Chinese)

李全胜，谢宗铭，张国丽，等．棉花黄萎病拮抗芽孢杆菌S12的筛选鉴定及拮抗机制的分析[J]．南京农业大学学报，2015，38(3)：402-408．

LI Q S，XIE Z M，ZHANG G L，et al．Screening and identification of antagonistic spore bacterium S12 against cotton Verticillium wilt and preliminary study on its antagonistic mechanisms[J]．Journal of Nanjing Agricultural University，2015，38(3)：402-408．(in Chinese)

程亮，游春平，肖爱萍．拮抗细菌的研究进展[J]．江西农业大学学报，2003，25(5)：732-737．

CHENG L，YOU C P，XIAO A P．Advance in the study on antagonistic bacteria[J]．Acta Agriculturae Universitis Jiangxiensis，2003，25(5)：732-737．(in Chinese)

孙广正，侯栋，岳宏忠，等．番茄早疫病菌拮抗细菌的筛选及其抑制作用[J]．草原与草坪，2015，35(1)：32-36+43．

SUN G Z，HOU D，YUE H Z，et al．Screening of bacteria antagonizing Alternaria solani and its inhibitory effects[J]．Grassland and Turf，2015，35(1)：32-36+43．(in Chinese)

杨星月，陆跃岭，金东林，等．抗番茄早疫病海洋细菌221-11的鉴定及其生防效果评价[J]．植物医学，2024，3(5)：18-27．

YANG X Y，LU Y L，JIN D L，et al．Identification and biocontrol evaluation of marine bacteria 221-11 on tomato early blight[J]．Plant Health and Medicine，2024，3(5)：18-27．(in Chinese)

黄曦，许兰兰，黄荣韶，等．枯草芽孢杆菌在抑制植物病原菌中的研究进展[J]．生物技术通报，2010，26(1)：24-29．

HUANG X，XU L L，HUANG R S，et al．Research advance in controlling plant diseases by Bacillus subtilis[J]．Biotechnology Bulletin，2010，26(1)：24-29．(in Chinese)

刘雪，穆常青，蒋细良，等．枯草芽孢杆菌代谢物质的研究进展及其在植病生防中的应用[J]．中国生物防治，2006，22(S1)：179-184．

LIU X，MU C Q，JIANG X L，et al．Research progress of the metabolic substances produced by Bacillus subtilis and their application on biocontrol of plant disease[J]．Chinese Journal of Biological Control，2006，22(S1)：179-184．(in Chinese)

朱洪磊，马超，郭洁心，等．芽孢杆菌抗菌肽调控基因的PCR检测及其抑菌活性[J]．山西农业科学，2020，48(3)：304-308．

ZHU H L，MA C，GUO J X，et al．PCR detection of regulation gene of antibacterial lipopeptide from Bacillus sp. and its antifungal activity[J]．Journal of Shanxi Agricultural Sciences，2020，48(3)：304-308．(in Chinese)

ZHOU L，SONG C X，LI Z B，et al．Antimicrobial activity screening of rhizosphere soil bacteria from tomato and genome-based analysis of their antimicrobial biosynthetic potential[J]．BMC Genomics，2021，22(1)：29．

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰